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GrainGenes Reference Report: JPY-158-929

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Reference	JPY-158-929
Title	Changes in the wheat Em 5 ' UTR affect reporter gene expression in vivo and in vitro
Journal	Journal of Plant Physiology
Year	2001
Volume	158
Pages	929-934
Author	Paradkar MR Marcotte WR
Abstract	Summary: The wheat Em gene is abundantly expressed in seed embryos during late embryogenesis and both transcriptional and post-transcriptional levels of control have been implicated. In this study, we have demonstrated that the wheat Em 5' untranslated region (UTR) is capable of enhancing expression levels both in vivo and in vitro when present as part of a chimeric synthetic mRNA. Due to enhancement in both systems, we attribute the bulk of the increase in expression to enhanced translation. Computer-aided analysis of the Em 5'UTR suggests that this sequence has the potential to form several stable secondary structures. When one of these structures, a predicted stem-loop, is deleted, expression is reduced in both the in vivo and in vitro assays suggesting that this region of the Em 5'UTR may be involved in enhanced expression
Keyword	[ Hide all but 1 of 34 ] abscisic acid assay assays beta-glucuronidase clone coupled in vitro transcription-translation embryo embryogenesis enhanced expression gene gene expression in vitro in vivo late embryogenesis messenger rna mrna nucleotide sequence promoter protein protoplast region reporter reporter gene secondary structure seed seed embryos sequence structure system transient gene expression translation untranslated region vitro viviparous1 vp1

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