Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: MBR-5-53

[Submit comment/correction]

Reference	MBR-5-53
Title	Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe.
Journal	Molecular Breeding
Year	1999
Volume	5
Pages	53-63
Author	Altpeter F [ Show all 6 ]
Abstract	Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects.
External Databases	Agricola: 2010-58260
Keyword	[ Hide all but 1 of 22 ] bioassay biolistics developmental stages gene expression gene transfer genetic transformation hordeum vulgare inhibitor insect insect resistance messenger rna pest resistance resistance sitotroga cerealella stored products pests survival transgenic transgenic plant transgenic wheat triticum aestivum trypsin trypsin inhibitors

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.