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GrainGenes Reference Report: MGG-248-592

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Reference
MGG-248-592
Title
The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco
Journal
Molecular and General Genetics
Year
1995
Volume
248
Pages
592-598
Author
Diaz I
Royo J
O'Connor A
Carbonero P
Abstract
Tissue-specific expression of the gene coding for trypsin inhibitor BTI-CMe in barley (Itr1) occur during the first half of endosperm development In transgenic tobacco, the Itr1 promoter drives expression of the beta-glucuronidase reporter gene not only in developing endosperm but also in embryo, cotyledons and the meristematic intercotyledonary zone of germinating seedlings A promoter fragment extending 343 bp upstream of the translation initiation ATG codon was sufficient for full transgene expression, whereas, the proximal 83 bp segment of the promoter was inactive Possible reasons for the differences in expression patterns are discussed
Keyword
[ Hide all but 1 of 19 ]
beta-glucuronidase
cotyledons
endosperm
gene expression
gene transfer
histoenzymology
hordeum vulgare
itr1 gene
itr1-promoter
nicotiana tabacum
plant embryos
promoters
recombinant-dna
reporter genes
seed
seedling
structural genes
transgenic-plants
trypsin-inhibitors

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