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GrainGenes Reference Report: MPM-12-728

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Reference	MPM-12-728
Title	Characterization of a host-specific protein toxin (Ptr ToxB) from Pyrenophora tritici-repentis
Journal	Molecular Plant Microbe Interactions
Year	1999
Volume	12
Pages	728-732
Author	Strelkov SE Lamari L Ballance GM
Abstract	Summary: Pyrenophora tritici-repentis, the causal agent of tan spot of wheat, differentially induces tan necrosis and/or chlorosis in wheat. A chlorosis-inducing, host-specific toxin, termed Ptr ToxB (formerly Ptr chlorosis toxin), was purified from the culture filtrates of a race 5 isolate of P. tritici-repentis. Partial purification was performed by 25 to 80% ammonium sulfate precipitation and passage through a carboxymethyl-Sephadex C-25 cation exchange column. Final purification was performed by fast performance liquid chromatography, with a Mono S HR 5/5 cation exchanger, followed by size fractionation on a Superose 12 HR 10/30 column. The toxin was shown to be proteinaceous in nature, and purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular mass of Ptr ToxB was determined to be 6.61 kDa. The amino acid composition and partial N terminus amino acid sequence of the toxin were also obtained. Ptr ToxB was found to be heat stable, maintaining full toxic activity after 1 h at 55 degrees C. Infiltration of toxin concentrations as low as 14 nM produced chlorosis on susceptible cultivars
Keyword	[ Hide all but 1 of 21 ] amino acid amino acid sequence ammonium chlorosis cultivar culture filtrates electrophoresis heat heat stability host range molecular weight mycotoxins necrosis performance protein composition purification pyrenophora tritici repentis sequence susceptibility symptoms triticum aestivum

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