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GrainGenes Reference Report: MPM-14-832

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Reference	MPM-14-832
Title	Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat
Journal	Molecular Plant-Microbe Interactions
Year	2001
Volume	14
Pages	832-838
Author	Oldach KH Becker D Lorz H
Abstract	Summary: Three cDNAs encoding the antifungal protein Ag-AFP from the fungus Aspergillus giganteus, a barley class II chitinase and a barley type I RIP, all regulated by the constitutive Ubiquitin1 promoter from maize, were expressed in transgenic wheat. In 17 wheat lines, stable integration and inheritance of one of the three transgenes has been demonstrated over four generations. The formation of powdery mildew (Erysiphe graminis f. sp. tritici) or leaf rust (Puccinia recondita f. sp. tritici) colonies was significantly reduced on leaves from afp or chitinase II- but not from rip I-expressing wheat lines compared with nontransgenic controls. The increased resistance of afp and chitinase II lines was dependent on the dose of fungal spores used for inoculation. Heterologous expression of the fungal afp gene and the barley chitinase II gene in wheat demonstrated that colony formation and, thereby, spreading of two important biotrophic fungal diseases is inhibited approximately 40 to 50% at an inoculum density of 80 to 100 spores per cm2
External Databases	Pubmed: 11437256
Keyword	[ Hide all but 1 of 52 ] antifungal antifungal protein aspergillus aspergillus-giganteus barley beta-1,3-glucanase bombardment cdna chitinase chitinase gene defense density disease diseases erysiphe erysiphe graminis fungal fungal diseases fungal spores fungus gene generation giganteus graminis heterologous expression ii gene inheritance inoculation inoculum integration leaf rust line maize mildew pathogenesis-related protein plant science powdery mildew promoter protein puccinia puccinia recondita recondita resistance ribosome-inactivating proteins rust sp stable integration tobacco transgene transgenic transgenic wheat tritici

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