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GrainGenes Reference Report: PBR-118-395

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Reference	PBR-118-395
Title	Chromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat
Journal	Plant Breeding
Year	1999
Volume	118
Pages	395-398
Author	Jimenez M Dubcovsky J Jiminez M
Abstract	Summary: This study used cytogenetic stocks to investigate the chromosomal location of genes responsible for polyphenol oxidase (PPO) activity in common and durum wheat seeds. Substitution lines of chromosome 2A of hexaploid varieties 'Cheyenne', 'Thatcher' and 'Timstein' in 'Chinese Spring' showed significantly higher PPO activity than all other substitution lines of the same variety, with the exception of substitutions of 'Cheyenne' chromosome 3A and 'Thatcher' chromosome 4B. Substitution lines of chromosome 2A of Triticum turgidum var. dicoccoides and of chromosome 2D of 'Chinese Spring' into the tetraploid variety 'Langdon' showed a significant increase in PPO activity relative to all other substitution lines in Langdon. The gene(s) responsible for high PPO activity in chromosome 2D from 'Chinese Spring' was mapped on the long arm within a deletion that represents 24% of the distal part of the arm. This study shows that genes located in homoeologous group 2 play a major role in the activity of PPO in wheat
Keyword	[ Hide all but 1 of 12 ] catechol oxidase chromosome maps deletion enzyme activity gene location genetic mapping seeds substitution lines triticum triticum aestivum triticum dicoccoides triticum turgidum

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