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GrainGenes Reference Report: PBR-119-383

Reference	PBR-119-383
Title	Molecular and chemical analysis of beta-amylase-less mutant barley in Tibet
Journal	Plant Breeding
Year	2000
Volume	119
Pages	383-387
Author	Kaneko T Kihara M Ito K Takeda K
Abstract	Summary: The molecular and biochemical aspects of Tibetan beta-amylase-less mutant barley were investigated. An enzyme assay showed that there were eight beta-amylase-less mutant lines in the Tibetan landrace barley. Following Southern analysis and polymerase chain reaction, it is suggested that these mutants result from the same insert mutation in the latter part of the beta-amylase structural gene. The alpha-glucosidase activity level of the mutant line was high in the initial period of germination, and the glucose ratio in the sugar conformation was remarkably high in the germinated seed of the mutant line. These results suggest that this beta-amylase-less mutant line could be a new breeding material for beer production
Keyword	[ Hide all but 1 of 23 ] alpha amylase assay barley beer beer quality beta-amylase breeding conformation dna dna marker enzyme gene germination glucose hordeum vulgare landrace mutant mutation polymerase polymerase chain reaction seed sugar

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