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GrainGenes Reference Report: PBR-119-389

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Reference	PBR-119-389
Title	Molecular mapping of the leaf rust resistance gene Rph7 in barley
Journal	Plant Breeding
Year	2000
Volume	119
Pages	389-392
Author	Graner A [ Show all 6 ]
Abstract	Summary: Leaf rust of barley, caused by Puccinia hordei Otth, is an important foliar disease in most temperate regions of the world. Sixteen major leaf rust resistance (Rph) genes have been described from barley, but only a few have been mapped. The leaf rust resistance gene Rph7 was first described from the cultivar 'Cebada Capa' and has proven effective in Europe. Previously mapped restriction fragment length polymorphism (RFLP) markers have been used to determine the precise location of this gene in the barley genome. From the genetic analysis of a 'Bowman'/'Cebada Capa' cross, Rph7 was mapped to the end of chromosome 3HS, 1.3 recombination units distal to the RFLP marker cMWG691. A codominant cleaved amplified polymorphic site (CAPS) marker was developed by exploiting allele-specific sequence information of the cMWG691 site and adjacent fragments of genomic DNA. Based on the large amount of polymorphism present in this region, the CAPS marker may be useful for the marker-assisted selection of Rph7 in most diverse genetic backgrounds
Keyword	[ Hide all but 1 of 39 ] barley barley genome bulked segregant analysis chromosome codominant disease dna europe fragment length polymorphism gene genetic analysis genetic background genome genomic dna hordei hordeum vulgare leaf rust leaf rust resistance linkage lr10 mapping marker marker assisted selection molecular mapping polymorphism puccinia puccinia hordei recombination resistance resistance gene rflp rflp marker rust rust resistance rust resistance gene segregant analysis selection sequence site

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