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GrainGenes Reference Report: PBR-121-400

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Reference	PBR-121-400
Title	Identifying AFLP and microsatellite markers for vernalization response gene Vrn-B1 in hexaploid wheat using reciprocal mapping populations
Journal	Plant Breeding
Year	2002
Volume	121
Pages	400-406
Author	Barrett B Bayram M Kidwell K
Abstract	Summary: Marker-assisted selection may be useful for combining specific vernalization response (Vrn) alleles into a single wheat genotype for yield enhancement; however, DNA markers are only available for two of the three genes identified to date. The objectives of this study were to investigate reciprocal effects on days to heading using F2 populations generated by cross-hybridizing near-isogenic lines (NILs) carrying spring (Vrn-B1; TDB) and winter (vrn-B1; TDC) alleles, and to identify markers linked to Vrn-B1 through genetic linkage analysis. Heading data were recorded for 91 and 89 progeny from reciprocal mapping populations TDB/TDC and TDC/TDB, respectively, and significant (P < 0.0001) reciprocal and dominance effects were detected. Among 207 amplified fragment length polymorphisms primer pairs and seven wheat microsatellite markers screened, two and one, respectively, were linked distally to Vrn-B1 on wheat chromosome 5BL. Microsatellite Xgwm408 was most closely linked to Vrn-B1 at 3.9 and 1.1 cM in the TDB/TDC and TDC/TDB map, respectively. Reciprocal differences in recombination distances emphasize the importance of female parent choice when generating mapping populations. Molecular markers are now available for three Vrn loci in wheat
Keyword	[ Hide all but 1 of 37 ] aflp allele amplified fragment length polymorphism bread wheat chromosome chromosome 5a dna dna methylation dominance ear emergence time f-2 population fragment length polymorphism gene genetic mapping genotype hexaploid hexaploid wheat linkage locus major genes map mapping maternal effects microsatellite marker molecular markers near-isogenic line polymorphism powdery mildew quantitative trait locus recombination resistance gene rflp marker selection triticum aestivum vernalization winter habit yield

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