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GrainGenes Reference Report: PBR-122-99

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Reference	PBR-122-99
Title	Genetic analysis of tissue culture traits in barley cv. 'Lenins'
Journal	Plant Breeding
Year	2003
Volume	122
Pages	99-104
Author	Rikiishi K Matsuura T Maekawa M Noda K Takeda K
Abstract	Summary: The barley cultivar 'Lenins' was found to be a genotype showing high shoot regeneration ability in cultures derived from immature embryos. Five cultivars different from 'Lenins' in shoot regeneration ability were reciprocally crossed with 'Lenins' and the inheritance of tissue culture traits was investigated. F2 plants showed continuous distributions in callus growth and percentage of shoot regeneration, suggesting that these traits were controlled by polygenes. The F2 population, derived from a cross between 'Lenins' and '6721', showed a monogenic segregation for the number of regenerated shoots, and the segregation ratio fitted 1:2:1. Tissue culture traits of 'Lenins' were controlled by several genes, whereas the number of regenerated shoots related to the efficiency of shoot regeneration is controlled by one major gene
Keyword	[ Hide all but 1 of 26 ] barley callus callus formation ability callus growth culture embryo embryogenesis f-2 population gene genotype growth inheritance invitro l anther culture maize major gene plant regeneration polygenes population regeneration rice segregation shoot shoot regeneration tissue triticum aestivum

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