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GrainGenes Reference Report: PBR-122-116

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Reference	PBR-122-116
Title	Microsatellite monitoring of recombination around the Vrn-B1 locus of wheat during early backcross breeding
Journal	Plant Breeding
Year	2003
Volume	122
Pages	116-119
Author	Salina E Dobrovolskaya O Efremova T Leonova I Roder MS
Abstract	Summary: The length of chromosomal segments retained around the Vrn-B1 gene controlling sensitivity to vernalization in wheat (Triticum aestivum L.) was studied in the first and third backcrosses by using microsatellite markers. Eleven polymorphic markers located on chromosome 5B were used for microsatellite analysis. It was shown in the first backcross that plants with a donor segment around the gene of interest not longer than 50% of chromosome 5B could be selected. When selection is not molecular-marker assisted, the length of the chromosomal donor segment with the target gene may reach 94% of chromosome 5B even in plants of the third backcross generation. The considerable length differences in the 5B microsatellite loci between the winter and spring lines of wheat studied indicate that these markers are promising in marker-assisted backcrossing or marker-assisted selection for the Vrn-B1 gene using different combinations of Spring and Winter genotypes
Keyword	[ Hide all but 1 of 29 ] 5b chromosome allele backcross backcrossing breeding chromosome difference donor gene generation isogenic lines linkage drag marker marker-assisted backcrossing marker-assisted selection microsatellite microsatellite loci molecular marker monitoring near-isogenic line recombination selection sensitivity spring triticum triticum aestivum vernalization vrn-b1 winter

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