GrainGenes Reference Report: PBR-122-120
Reference
PBR-122-120
Title
Genetic polymorphisms at Gli-D-t gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis
Journal
Plant Breeding
Year
2003
Volume
122
Pages
120-124
Author
Yan YM Hsam SLK Yu JZ Jiang Y Zeller FJ
Abstract
Summary: Gliadin variation at Gli-Dt1 and Gli-Dt2 loci in 198 Aegilops tauschii accessions was studied by acid polyacrylamide gel electrophoresis (A-PAGE) and capillary electrophoresis (CE). High genetic polymorphisms were found at both gliadin coding loci, revealing a total of 184 and 169 gliadin variants at the Gli-Dt1 and Gli-Dt2 loci, respectively. In particular, 12 gliadin blocks encoded by different alleles were apparently expressed and readily identified in six synthetic hexaploids produced by hybridization between Triticum durum and Ae. tauschii accessions. Compared with Ae. tauschii ssp. eusquarrosa, the gliadin profile of the D genome in Ae. tauschii ssp. strangulata more resembles that of T. aestivum, supporting the view that the subspecies strangulata is the most likely progenitor of bread wheat. Capillary electrophoresis analysis showed that the method is capable of separating and characterizing gliadins with speed, in high resolution using small sample amounts, and is well-suited to detect protein alleles and to identify desirable genotypes in wheat quality improvement
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-122-120
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||