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GrainGenes Reference Report: PCT-75-35

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Reference	PCT-75-35
Title	The effect of osmotic potential on anther culture in spring wheat (Triticum aestivum)
Journal	Plant Cell, Tissue and Organ Culture
Year	2000
Pages	35-40
Author	Kang TJ Yang MS Deckard EL
Abstract	Summary: This study was conducted to determine the effect of osmotic potential in a modified 85D12 medium on both callus induction and plant regeneration in the anther culture of two wheat genotypes, cv. Chris and cv. Pavon. Altering the medium osmotic potential by changing the carbohydrate source and concentration or by adding a non-metabolized osmoticum appeared to have the greatest potential for improving anther-derived green plant production. The medium osmotic potentials were varied (-0.67 to -2.30 MPa) by altering sucrose and PEG concentration. Both osmotica affected callus production, with -0.9 to -1.4 MPa media producing the most calluses. Callus production depended on genotype and osmoticum. Only PEG concentration affected green plant regeneration. The greatest number of green plants (11.5 plants per 100 anthers in cv. Chris) was obtained with 0.0125 M of PEG. This experiment suggested that a low level of PEG in the medium was beneficial for producing green plants from wheat anthers
Keyword	[ Hide all but 1 of 21 ] anther anther culture anthers concentration cultivar culture media dose response genetic variation green plant regeneration in vitro regeneration induction media methodology micropropagation osmotic pressure polyethylene glycol regeneration spring wheat sucrose triticum triticum aestivum

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