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GrainGenes Reference Report: PGR-17-7

Reference	PGR-17-7
Title	Development of a one-step enzyme immunoassay for the quantitation of gibberellin A3 in barley seeds
Journal	Plant Growth Regulation
Year	1995
Volume	17
Pages	7-13
Author	Bounaix C Doumas P
Abstract	The development of a sensitive and specific enzyme immunoassay for GA(3) is reported This method was based on the use of peroxidase labelled GA(3) and immobilized antibodies In order to obtain a rapid immunoassay, several steps of purification were analyzed to show their necessity Barley seed extracts were assayed at different steps of purification to exhibit the effect of extract components on the assay It was demonstrated that HPLC had to be performed when a selective quantitation of GA(3) was required This assay allowed GA(3) to be measured with reproducibility as its unmethylated form and the quantitation of GA(3) in barley seeds with this enzyme immunoassay was correlated to a GC-MS method
Keyword	[ Hide all but 1 of 9 ] gibberellins hordeum vulgare immunoassay peroxidase plant composition purification quantitative-analysis rapid-methods seed

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