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GrainGenes Reference Report: PHY-85-319

Reference	PHY-85-319
Title	Polymerase chain reaction assays for the detection of Stagonospora nodorum and Septoria tritici in wheat
Journal	Phytopathology
Year	1995
Volume	85
Pages	319-324
Author	Beck J Ligon J
Abstract	Polymerase chain reaction (PCR) primers have been developed that can distinguish important wheat pathogenic fungal species and detect their DNA in infected wheat leaf tissues. Septoria tritici (teleomorph Mycosphaerella graminicola) and Stagonospora nodorum = Septoria nodorum (teleomorph Phaeosphaeria nodorum), are agronomically significant pathogens of wheat, causing wheat leaf and glume blotch, respectively. Primers made to conserved sequences of the ribosomal DNA were used to PCR amplify and clone the internal transcribed spacer (ITS) regions from S. nodorum and S. tritici. The ITS regions were sequenced, the sequences were aligned, and species-specific PCR primers were designed to the polymorphic regions. These ITS-derived primers were used to amplify specific fragments from the fungi from which they were designed, thus allowing the detection of the pathogens. The primers also successfully amplified similar-sized fragments from wheat tissues infected with the respective fungi.
Keyword	[ Hide all but 1 of 15 ] detection diagnostic-techniques genetic markers genetic polymorphism internal-transcribed-spacers moleculor-sequence-data mycosphaerella-graminicola nucleotide sequence pathogenicity pathotype polymerase chain reaction ribosomal dna stagonospora symptoms triticum aestivum

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