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GrainGenes Reference Report: PHY-90-666

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Reference	PHY-90-666
Title	Antifungal activity toward Fusarium culmorum in soluble wheat extracts
Journal	Phytopathology
Year	2000
Volume	90
Pages	666-671
Author	Doohan FM Mentewab A Nicholson P
Abstract	Summary: This study investigated antifungal activity in soluble extracts from seed of a range of wheat cultivars differing in susceptibility to Fusarium head blight. Antifungal activity was assessed in terms of beta-D-glucuronidase (GUS) activity of a Fusarium culmorum GUS transformant using a sensitive laboratory assay. Significant antifungal activity was detected in seed extracts from WEK0609, CM 820036, and Arina. Initial characterization of the Arina seed extract indicated that it contained antifungal proteinaceous compounds. The Arina extract yielded two (60 and 80%) ammonium sulfate fractions containing inhibitory compounds. Gel filtration chromatography and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of antifungal fractions showed that the antifungal activities detected in the Arina 60 and 80% ammonium sulfate fractions were associated with putative proteinaceous compounds with apparent molecular masses of approximately 60 and 28 kDa, respectively
Keyword	[ Hide all but 1 of 32 ] ammonium antifungal antifungal activity antifungal properties assay blight characterization chromatography culmorum cultivar disease resistance electrophoresis fusarium fusarium culmorum fusarium head blight gel electrophoresis genetic variation gibberella zeae gus head blight molecular masses pathogenicity plant extracts plant pathogenic fungi seed seeds sodium sodium dodecyl sulfate-polyacrylamide gel sulfate susceptibility triticum aestivum wheat cultivar

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