Tube label

Microliters of enzyme solution added to tube

Hind III quantity in tube (units)

Final HindIII concentration in tube (units/ml)*

a

0

0

0

b

1 µl of Dilution 2

0.2

0.05

c

2.5 µl of Dilution 2

0.5

0.125

d

5.0 µl of Dilution 2

1.0

0.25

e

7.5 µl of Dilution 2

1.5

0.375

f

1.0 µl of Dilution 1

2.0

0.5

g

1.5 µl of Dilution 1

3.0

0.75

h

2.0 µl of Dilution 1

4.0

1.0

i

2.5 µl of Dilution 1

5.0

1.25

j

5.0 µl of Dilution 1

10.0

2.5

k

2.5 µl of undiluted HindIII

50.0

12.5