FIGURE 11.2 - Photograph of a partially reconstructed gel. All gel pieces, except for those containing DNA to be used in the second size selection, were stained, placed on a UV light box, reassembled, and photographed. Eight plugs containing Gossypium raimondii nuclear DNA were loaded into the slot well. The high density of DNA in the plugs (prepared using OPTION X, CHAPTER 7) is reflected in the intense ethidium bromide staining of the G. raimondii DNA. The unstained portion of the gel (not shown) containing DNA between 100 and 350 kb was used in a second size selection.