FIGURE 16.2 - Hand-picked libraries. (a) A sterile toothpick is used to stab a white colony on an X/I/C plate. (b) The end of the toothpick containing the colony is then inserted into the first well of a microtiter plate containing freezing medium. The toothpick is left in the well so that the well is not accidentally inoculated twice. (c) Other clones (on toothpicks) are placed in the remaining wells of the first row. Once the first row has been completely inoculated, toothpicks are removed one at a time from the wells. The other rows of the plate are inoculated in this manner until the plate has been completely inoculated. The lid is placed back on the plate, and the plate is set aside. (d) Inoculated plates are stacked in columns and incubated at 37°C overnight.