FIGURE 1.1 - pBeloBAC11. The genes parA, parB, and parC are required for partitioning. Additionally, parB and parC are required for incompatibility with other F factors. The repE gene encodes a protein essential for replication from the oriS. A chloramphenicol resistance gene (CM^R) has been incorporated for antibiotic selection of transformants. pBeloBAC11 has a polycloning site with recognition sequences for three different restriction enzymes (HindIII, BamHI and SphI). The polycloning site is located within the lacZ gene allowing identification of recombinants by alpha-complementation. The figure above is based on Figure 1 from Choi and Wing (1999).