FIGURE A.2 - Loading an electroporator cuvette. (a) A cuvette shown from the front. To facilitate understanding of the process of cuvette loading, the front plastic of the cuvette has been made transparent in b-d. (b) The inner left and right sides of the cuvette are covered by unconnected metal liners. Each liner has a distinctive knob protruding inwards directly opposite the knob on the other liner. (c) A combination of competent cells and ligate are placed between the two knobs. (d) Closing the lid of the cuvette brings each metal liner into contact with one of the two electrode pins in the lid.