FIGURE B.4 - Electroelution. The cuvette rack is placed into the main buffer tank. 1X TAE is added to the main tank until the assembly joints are covered in buffer. Likewise, 1X TAE is added to the upper buffer tank. Using a stir bar, the buffer in the main tank is gently agitated. The electrode plug is inserted into the BioRad power source (not shown). In general, each cuvette requires 10 mA of power (e.g., if three cuvettes are used, the power should be set at 30 mA). Perform electroelution for two hours (see APPENDIX B for details).