FIGURE B.5 - Recovery of DNA. Once electroelution is complete, lift the cuvette holder from the main buffer tank. Remove buffer in the upper buffer tank using a sterile Pasteur pipet. (a) Using the pipet, gently remove all buffer above the surface of the Miracloth at the bottom of each glass tube. PERFORM THIS STEP SLOWLY AND CAREFULLY! IF THE BUFFER IS ASPIRATED TOO RAPIDLY OR IF SUCTION IS APPLIED TO THE MIRACLOTH ITSELF, DNA WILL BE PULLED OUT OF THE COLLECTION CUP! (b) Remove a cuvette from the cuvette holder. Gently remove the glass tube, Miracloth, and agarose cube from the assembly joint. A droplet of liquid containing the DNA should be visible at the bottom of the collection cup. (c) Using a wide-orifice plastic pipet tip, slowly remove the DNA solution and place it in a sterile 1.5 ml microfuge tube. Approximately 0.4 ml of DNA solution should be recovered from each cuvette.