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GrainGenes Marker Report: Xwmc99-5B

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Locus	Xwmc99-5B
Type	Microsatellite
Chromosome	5B
Map	Ta-SSR-2004-5B 128.1
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC99
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc99 wmc99 wmc99-5B
Probe for Locus	WMC99
Locus	Xwmc99 Xwmc99-5B wmc99 wmc99-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7c3 Donor: Somers, Agriculture Canada Trimmed to insert at 5' end. 3' end not located.(GT)13 160 to 185. (2 loci? 1 polymorph)
Type	PCR SSR
PCR primers	Forward ATTAcAATTgcTTcAgTgAgTg at 51 length 22 bases, Tm 56 Reverse TcATgATcATTgTTATAAcggT at 187 length 22 bases, Tm 56
SSR size	158 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC99
Source Species	Triticum aestivum