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GrainGenes Marker Report: BARC227

Probe	BARC227
Locus	Xbarc227-4B Xbarc227
Remarks	Contains motif : (CT)13+6 SSR size is the band size in Chinese Spring BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCA CCG AAG CTC TAA TAC CAA ATG T 3' 5' GCG TTG AGG AGT GTT CTC TGT TCT GGA 3'
SSR size	192
Amplification Conditions	Annealing temperature = 58
Sequence	BV211830
Source Species	Triticum aestivum