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GrainGenes Marker Report: BE405552

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Probe	BE405552
Locus	HD012A21 HD020H15 D1.1_ctg4219 Sr35 XBE405552
Reference	Zhang W et al. (2010) Genetic Maps of Stem Rust Resistance Gene Sr35 in Diploid and Hexaploid Wheat Crop Science 50:2464-2474. Zhang W et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr35 MAS Wheat. Marker Assisted Selection in Wheat.
Type	Indel PCR
PCR primers	BE405552-F CACCATCTTCGTCACCATCA BE405552-R CACAGTGCAGCGAACAGAT
Amplification Conditions	Touch down from 63°C to 59°C (9 cycles of 20 sec, -0.5°C each) Amplification at 58°C (34 cycles of 20 sec each)
Source Species	Triticum aestivum