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GrainGenes Marker Report: BMAG0877

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Locus	Bmag0877
Type	Microsatellite
Chromosome	3H
Map	Barley-ConsensusFHB-3H 139.08 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 7 ]
Species	Hordeum vulgare
Probe	BMAG0877 Bmag877
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmag877
Probe for Locus	Bmag877
Locus	Bmag877 Bmag0877
Synonym	See also BMAG0877
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GA)15 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.77
Type	PCR SSR
PCR primers	5' AAAGCTCATGGTAGATCAAGA 3' 5' TAGTTTTCCCAAAAGCTTCTA 3'
SSR size	153
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare