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GrainGenes Marker Report: Bmac0040

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Locus	Bmac0040
Type	Microsatellite
Synonym	Synonym Bmac40
Chromosome	6H
Map	Barley, B73xCPI-6H 47.8 [ Show all 13 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.DON_6H_6
Map Data	Barley, Abiotic QTL Consensus [ Show all 13 ]
Species	Hordeum vulgare
Probe	Bmac0040 Bmac40
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318.
Possible Orthologs	Bmac40 Bmac0040a Bmac0040b
Probe for Locus	Bmac40
Locus	Bmac0040 Bmac40 Bmac0040b Bmac0040a
Synonym	Other name Bmac0040
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)20 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.89
Type	PCR SSR
PCR primers	5' AGCCCGATCAGATTTACG 3' 5' TTCTCCCTTTGGTCCTTG 3'
SSR size	236
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare