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GrainGenes Marker Report: Bmac0044

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Locus	Bmac0044
Type	Microsatellite
Chromosome	1H
Map	Barley-ConsensusFHB-1H 60.2 Hordeum-Consensus2007-SSR-1H 64.84 Hordeum-LxHs-1H 58
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Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0044 Bmac44
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)26 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	Bmac44
Locus	Bmac0044
Synonym	Other name Bmac0044
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)26 Quality = 5: Bands of unexpected size or additional bands. Very weak bands or no amplification products
Type	PCR SSR
PCR primers	5' TGCCATATCATCTTTCTCAGACA 3' 5' GGTCAAGGATAGTTGGGTAATCC 3'
SSR size	199
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare