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GrainGenes Marker Report: Bmac0096

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Locus	Bmac0096
Type	Microsatellite
Synonym	Synonym Bmac96
Chromosome	5H
Map	Barley, B73xCPI-5H 51.1 [ Show all 14 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 14 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0096 Bmac96
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	Bmac96
Probe for Locus	Bmac96
Locus	Bmac0096 Bmac96
Synonym	Other name Bmac0096
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AT)6(AC)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' GCTATGGCGTACTATGTATGGTTG 3' 5' TCACGATGAGGTATGATCAAAGA 3'
SSR size	173
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare