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GrainGenes Marker Report: Bmac0218

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Locus	Bmac0218
Type	Microsatellite
Synonym	Synonym Bmac218
Chromosome	2H
Map	Hordeum-Consensus2005-SNP-2H 84.025 Hordeum-LiHs-SNP-2H 46.93 Hordeum-LxHs-2H 33 Hordeum-QTLConsensus-Abiotic-2H 84.03 Hordeum-QTLConsensus-Agronomic-2H 84.03
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Map Data	Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, Consensus 2005, SNP Barley, Lina x H.spont, SNP Barley, LxHs
Probe	Bmac218
Reference	Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Possible Orthologs	Bmac218 Bmac0218a Bmac218a-2H Bmac0218c
Probe for Locus	Bmac218
Locus	Bmac0218 Bmac0218a Bmac0218c Bmac218a-2H Bmac218
Synonym	Other name Bmac0218
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)14 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' ATTGCATTGATTAACTCCTACA 3' 5' GGGGGAATCTTTGTGTAAG 3'
SSR size	135
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare