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GrainGenes Marker Report: Bmac0273

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Locus	Bmac0273
Type	Microsatellite
Synonym	Synonym Bmac273
Chromosome	7H
Map	Barley, B73xCPI-7H 87 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 6 ]
Probe	Bmac0273 Bmac273
Reference	Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Possible Orthologs	Bmac273 [ Show all 7 ]
Probe for Locus	Bmac273
Locus	Bmac0273 [ Show all 8 ]
Synonym	Other name Bmac0273
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)20(AG)20 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.63
Type	PCR SSR
PCR primers	5' ACAAAGCTCGTGGTACGT 3' 5' AGGGAGTATTTCACCCTTG 3'
SSR size	186
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare