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GrainGenes Marker Report: Bmac0273f

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Locus	Bmac0273f
Type	Microsatellite
Map	Hv-Integrated2009-2H 65.3 Hv-Nure/Tremois-2H 79.9
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009 Barley, Nure x Tremois
Probe	Bmac273
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Nure x Tremois
Possible Orthologs	Bmac0273 [ Show all 7 ]
Probe for Locus	Bmac273
Locus	Bmac0273 [ Show all 8 ]
Synonym	Other name Bmac0273
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)20(AG)20 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.63
Type	PCR SSR
PCR primers	5' ACAAAGCTCGTGGTACGT 3' 5' AGGGAGTATTTCACCCTTG 3'
SSR size	186
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare