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GrainGenes Marker Report: Bmac0298

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Locus	Bmac0298
Type	Microsatellite
Synonym	Synonym Bmac298
Chromosome	4H
Map	Barley, B73xCPI-2H 71.7 [ Show all 8 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac0298 Bmac298
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)11 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	Bmac298
Probe for Locus	Bmac298
Locus	Bmac0298 Bmac298
Synonym	Other name Bmac0298
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)11 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' ACAACCACACAAGGTTGTC 3' 5' TTCCAGTTTCAGTCTCTTCAT 3'
SSR size	190
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 69C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 68C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 63C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare