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GrainGenes Marker Report: Bmac0310

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Locus	Bmac0310
Type	Microsatellite
Synonym	Synonym Bmac310
Chromosome	4H
Map	Barley, B73xCPI-4H 72.8 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 11 ]
Species	Hordeum vulgare
Probe	Bmac0310 Bmac310
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Possible Orthologs	Bmac310
Probe for Locus	Bmac310
Locus	Bmac0310 Bmac310
Synonym	Other name Bmac0310
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CT)11(AC)20 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' CTACCTCTGAGATATCATGCC 3' 5' ATCTAGTGTGTGTTGCTTCCT 3' CTACCTCTGAGATATCATGCC ATCTAGTGTGTGTTGCTTCCT
SSR size	176
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare