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GrainGenes Marker Report: Bmac113

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Locus	Bmac113
Type	Microsatellite
Synonym	Synonym Bmac0113
Chromosome	5H
Map	Barley-consensus-2003-5H 28.5 Hordeum-Consensus2006-DArT-5H 50.3 Hv-HNxH602-5H 75.2
	[ Show Nearby Loci ]
QTL with Locus Marker	QDP.AlSl-5H
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	Bmac113
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac113 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0113
Probe	Bmac113
Locus	Bmac0113 Bmac113
Synonym	Other name Bmac0113
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AT)7(AC)18 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.66
Type	PCR SSR
PCR primers	5' TCAAAAGCCGGTCTAATGCT 3' 5' GTGCAAAGAAAATGCACAGATAG 3'
SSR size	187
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare