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GrainGenes Marker Report: Bmac154

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Locus	Bmac154
Type	Microsatellite
Synonym	Synonym Bmac0154
Chromosome	1H
Map	Barley-consensus-2003-1H 81.6 Hordeum-Consensus2006-DArT-1H 78.5
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	Bmac154
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac154 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0154
Probe	Bmac154
Locus	Bmac0154 Bmac154
Synonym	Other name Bmac0154
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AT)19/(AC)6 Quality = 1: Single product, only weak stutter bands Null in H. spontaneum Canada Park.
Type	PCR SSR
PCR primers	5' CTGGGTGATGAATAGAGTTTC 3' 5' TATTCTTCAAAAGATGTTCTGC 3'
SSR size	130
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare