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GrainGenes Marker Report: Bmac156

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Locus	Bmac156
Type	Microsatellite
Chromosome	7H
Map	Barley-consensus-2003-7H 159.9 Hv-HNxH602-7H 282.5
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Map Data	Barley consensus 2003 Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	Bmac156
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Data Source	Langridge, Peter J.
Possible Orthologs	Bmac0156
Probe	Bmac156
Locus	Bmac0156 Bmac156
Synonym	Other name Bmac0156
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)22(AT)5 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.89
Type	PCR SSR
PCR primers	5' AACCGAATGTATTCCTCTGTA 3' 5' GCCAAACAACTATCGTGTAC 3'
SSR size	139
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare