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GrainGenes Marker Report: Bmac162

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Locus	Bmac162
Type	Microsatellite
Synonym	Synonym Bmac0162
Chromosome	7H
Map	Hordeum-Consensus2006-DArT-7H 87.4
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmac162
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmac162 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0162
Probe	Bmac162
Locus	Bmac0162 Bmac162
Synonym	Other name Bmac0162
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)15(CT)16 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' CATGTGTTGAAATCAGTTTTG 3' 5' CCCTCTCTCTCTCTCTCTCTC 3'
SSR size	187
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare