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GrainGenes Marker Report: Bmac167

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Locus	Bmac167
Type	Microsatellite
Synonym	Synonym Bmac0167
Chromosome	7H
Map	Barley-consensus-2003-7H 64.8 Hordeum-GxHN-SFNB-7H 25.5
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Map Data	Barley consensus 2003 Barley, GxHN, SFNB
Probe	Bmac167
Data Source	Langridge, Peter J.
Possible Orthologs	Bmac0167
Probe	Bmac167
Locus	Bmac0167 Bmac167
Synonym	Other name Bmac0167
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat AC)20 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park.
Type	PCR SSR
PCR primers	5' CATTTCCACTTCAAAATATCC 3' 5' CCAAAGTTTGAGTGCAGAC 3' CATTTCCACTTCAAAATATCC CCAAAGTTTGAGTGCAGAC
SSR size	184
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare