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GrainGenes Marker Report: Bmac175

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Locus	Bmac175
Type	Microsatellite
Synonym	Synonym Bmac0175a Bmac0175b Bmac0175c
Probe	Bmac175
Possible Orthologs	Bmac0175a Bmac0175b Bmac0175c
Probe	Bmac175
Locus	Bmac0175c Bmac0175a Bmac0175b Bmac175
Synonym	Other name Bmac0175
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)12 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type	PCR SSR
PCR primers	5' CTACACCCCTACCATATAAACA 3' 5' CCTCCCCACATACCTTGT 3'
SSR size	155
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare