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GrainGenes Marker Report: Bmac181

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Locus	Bmac181
Type	Microsatellite
Synonym	Synonym Bmac0181
Chromosome	4H
Map	Barley-consensus-2003-4H 26.9 Hordeum-Consensus2006-DArT-4H 67.5
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT
Probe	Bmac181
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac181 in the Barley, Consensus 2006 DArT maps = 3
Possible Orthologs	Bmac0181
Probe	Bmac181
Locus	Bmac0181 Bmac181
Synonym	Other name Bmac0181
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)20 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.75
Type	PCR SSR
PCR primers	5' ATAGATCACCAAGTGAACCAC 3' 5' GGTTATCACTGAGGCAAATAC 3' ATAGATCACCAAGTGAACCAC GGTTATCACTGAGGCAAATAC
SSR size	177
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare