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GrainGenes Marker Report: Bmac187-7H

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Locus	Bmac187-7H
Type	Microsatellite
Map	Hv-DxR-Stein-7H 56.06
	[ Show Nearby Loci ]
Map Data	Barley, OWB, Stein2006
Probe	Bmac187
Possible Orthologs	Bmac0187 Bmac187
Probe for Locus	Bmac187
Locus	Bmac0187 Bmac187-7H Bmac187
Synonym	Other name Bmac0187
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)13(AT)9 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' GCTCTCTCTCAGAAAAATGAA 3' 5' GAATTATTCTAGGGCTGTGAA 3' GCTCTCTCTCAGAAAAATGAA GAATTATTCTAGGGCTGTGAA
SSR size	177
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare