Query (optional)   in Class  

GrainGenes Marker Report: Bmac192

[Submit comment/correction]

Probe
Bmac192
Locus
Bmac0192c
Bmac0192b
Bmac0192d
Bmac0192a
Bmac0192
Synonym
Other nameBmac0192
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AC)25
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' GGGTGGTGTTGCTTAAAC 3'
5' TCAACATTCATACTACCACCA 3'
SSR size
165
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare