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GrainGenes Marker Report: Bmac213

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Locus	Bmac213
Type	Microsatellite
Synonym	Synonym Bmac0213
Chromosome	1H
Map	Barley-consensus-2003-1H 52.3 Hordeum-Consensus2006-DArT-1H 25.1 Hordeum-IxF-SSR-1H 18.6 Hordeum-SxM-SSR-1H 19.4 Hordeum-Zhedar2-FHB-QTL-1H 22.4
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Igri x Franka, SSR Barley, Steptoe x Morex, SSR Barley, Zhedar2, FHB QTL
Probe	Bmac213
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmac213 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0213
Probe	Bmac213
Locus	Bmac0213 Bmac213
Synonym	Other name Bmac0213
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	5' ATGGATGCAAGACCAAAC 3' 5' CTATGAGAGGTAGAGCAGCC 3' ATGGATGCAAGACCAAAC CTATGAGAGGTAGAGCAGCC
SSR size	168
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare