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GrainGenes Marker Report: Bmac216

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Locus	Bmac216
Type	Microsatellite
Synonym	Synonym Bmac0216
Chromosome	2H
Map	Hordeum-Consensus2006-DArT-2H 92.1
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmac216
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmac216 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0216
Probe	Bmac216
Locus	Bmac0216 Bmac216
Synonym	Other name Bmac0216
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)5 Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type	PCR SSR
PCR primers	5' GTACTATTCTTTGCTTGGGC 3' 5' ATACACATGTGCAAAACCATA 3'
SSR size	190
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare