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GrainGenes Marker Report: Bmac222

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Locus	Bmac222
Type	Microsatellite
Synonym	Synonym Bmac0222
Probe	Bmac222
Possible Orthologs	Bmac0222
Probe	Bmac222
Locus	Bmac0222 Bmac222
Synonym	Other name Bmac0222
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)23 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park.
Type	PCR SSR
PCR primers	5' ATTTGAATGTCCAACAGAATC 3' 5' GGGACTAAAGCCCCTTAC 3'
SSR size	165
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare