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GrainGenes Marker Report: Bmac224

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Locus	Bmac224
Type	Microsatellite
Synonym	Synonym Bmac0224
Chromosome	7H
Map	Hordeum-Consensus2006-DArT-7H 87.4
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmac224
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmac224 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmac0224
Probe	Bmac224
Locus	Bmac0224 Bmac224
Synonym	Other name Bmac0224
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)5/(AC)5 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.2
Type	PCR SSR
PCR primers	5' GCATATATACCACCCTTGGT 3' 5' ATTCTTGATGGCTATAGCTTG 3'
SSR size	166
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare