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GrainGenes Marker Report: Bmac251

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Locus	Bmac251
Type	Microsatellite
Synonym	Synonym Bmac0251
Chromosome	6H
Map	Barley-consensus-2003-6H 71.8 Hordeum-Zhedar2-FHB-QTL-6H 129
	[ Show Nearby Loci ]
QTL with Locus Marker	QDon.dah-6H.3 QFhs.dah-6H.2
Map Data	Barley consensus 2003 Barley, Zhedar2, FHB QTL
Probe	Bmac251
Data Source	Langridge, Peter J.
Possible Orthologs	Bmac0251
Probe	Bmac251
Locus	Bmac0251 Bmac251
Synonym	Other name Bmac0251
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)12A(AC)13 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CAGATCTCAACAAACACACC 3' 5' ATTCACTATAAACATATGGGTCC 3'
SSR size	185
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare