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GrainGenes Marker Report: Bmac282

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Locus	Bmac282
Type	Microsatellite
Synonym	Synonym Bmac0282a Bmac0282b
Probe	Bmac282
Possible Orthologs	Bmac0282a Bmac0282b
Probe	Bmac282
Locus	Bmac0282a Bmac0282b Bmac282
Synonym	Other name Bmac0282
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)5 Quality = 1: Single product, only weak stutter bands
Type	PCR SSR
PCR primers	5' CACACATACCACGCATGT 3' 5' ATGTAAAATGGACGTATCACC 3'
SSR size	195
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare